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Saturday, April 29, 2017

UNKNOWN FUNGUS FOUND GROWING ON INTERNATIONAL SPACE STATION - It is clogging waterlines and spreading on walls and surfaces - NASA will investigate

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Those of you who remember the film "Alien" know it's bad news to find an uninvited guest on board a spacecraft. 
  • Astronauts have reported funding strange microbial growths on walls and surfaces, and it has even clogged waterlines
  • Two instruments onboard will be used to analyse it in orbit
NASA is set to use a radical new 'tricorder' DNA sequencer to work out what a mysterious fungus found growing on the International Space Station is.

Astronauts have reported funding the strange microbial growths on walls and surfaces, and it has even clogged waterlines. 
Now, two instruments onboard will be used to analyse it in orbit, allowing mission controllers to work out how to deal with it.   Currently, the only way to identify contaminants is to take a sample and send it back to Earth.
 
Continue reading

Doing battle in "Alien"
'We have had contamination in parts of the station where fungi was seen growing or biomaterial has been pulled out of a clogged waterline, but we have no idea what it is until the sample gets back down to the lab,' said Sarah Wallace, NASA microbiologist and the project's principal investigator at the agency's Johnson Space Center in Houston.
 
'On the ISS, we can regularly resupply disinfectants, but as we move beyond low-Earth orbit where the ability for resupply is less frequent, knowing what to disinfect or not becomes very important,' said Wallace.
 
The new technique will marry two pieces of existing spaceflight technology, miniPCR and theMinION, to allow for the first unknown biological samples to be prepared, sequenced and then identified in space.  Developed in partnership by NASA's Johnson Space Center and Boeing, this ISS National Lab sponsored investigation  
NASA astronaut Kate Rubins poses for a picture with the minION device during its first test. Astronauts have reported funding the strange microbial growths on walls and surfaces, and it has even clogged waterlines, and will use the machine to analyse itNASA astronaut Kate Rubins poses for a picture with the minION device during its first test. Astronauts have reported funding the strange microbial growths on walls and surfaces, and it has even clogged waterlines, and will use the machine to analyse it
 

 

 
What the coupling of these different devices is doing is allowing us to take the lab to the samples, instead of us having to bring the samples to the lab,' said Aaron Burton, NASA biochemist and Genes in Space-3 co-investigator.  Crew members will collect a sample from within the space station to be cultured aboard the orbiting laboratory. 
 
The sample will then be prepared for sequencing, in a process similar to the one used during the Genes in Space-1 investigation, using the miniPCR and finally, sequenced and identified using the MinION device.
 
'The ISS is very clean,' said Sarah Stahl, microbiologist and project scientist.  'We find a lot of human-associated microorganisms - a lot of common bacteria such as Staphylococcus and Bacillus and different types of familiar fungi like Aspergillus and Penicillium.'
 
In addition to identifying microbes in space, this technology could be used to diagnose crew member wounds or illnesses in real time, help identify DNA-based life on other planets and help with other investigations aboard the station.
 
'The Genes in Space-3 process will increase the scientific capacity of the ISS by facilitating state-of-the-art molecular biology research for both current and next generation ISS researchers,' said Kristen John, NASA aerospace engineer and Genes in Space-3 project engineer. 
 
'The team has put a strong focus on generating a spaceflight-certified catalog of general laboratory items and reagents, and developing common methods and easily customizable reaction conditions for miniPCR and the MinION to enable other ISS researchers to use this technology.'
 
This process will give scientists on the ground real-time access to the experiments going on in space, allowing for more accuracy and a more efficient use of the time on the space station.
 
'If you could get a snapshot of the molecular signatures of your research as it was occurring on the ISS, how would you change your experiment?' said Wallace. 
 
'Would you change your time points? Provide a different nutrient? Alter growth conditions? You can imagine how, if you had that data, you could adjust your experiment to enhance the insight being gained.' 
 
Rubins, who studied Ebola and other deadly viruses before becoming an astronaut, will become the first virus-hunter in space, she will attempt to complete the first full-blown DNA decoding, or 'sequencing,' in orbit using this handhelp device.  
 
'There is huge power consumption at play with those as well.'    The compact biomolecule sequencer has minimal moving parts and plugs directly into a laptop or tablet, which supplies power to the device and collects the sequencing data.



HOW THE TRICORDER WORKS


The tiny, plug and play sequencer – about the size of a large candy bar – is diminutive compared to the large microwave-sized sequencers used on Earth.
 
'Most sequencers in Earth-based labs involve optics, fluorescence, lasers and other vibration sensitive components that are not suited for spaceflight or microgravity,' said Nasa Microbiologist and Project Manager Sarah Castro.
Rubins, who studied Ebola and other deadly viruses before becoming an astronaut, will become the first virus-hunter in space, she will attempt to complete the first full-blown DNA decoding, or 'sequencing,' in orbit using this handhelp device.
 

SEQUENCING IN  SPACE 

  • When NASA astronaut Kate Rubins sequenced DNA aboard the International Space Station in 2016, it was a game changer. That first-ever sequencing of DNA in space was part of the Biomolecule Sequencer investigation.
  • The miniPCR (polymerase chain reaction) device was first used aboard the station during the Genes in Space-1, and, soon to be Genes in Space-2 investigations, student-designed experiments in the Genes in Space program. Genes in Space-1 successfully demonstrated the device could be used in microgravity to amplify DNA, a process used to create thousands of copies of specific sections of DNA. The second investigation arrived at the space station on April 22, and will be tested this summer. 
  • Next came the Biomolecule Sequencer investigation, which successfully tested the MinION's ability to sequence strands of Earth-prepared DNA in an orbiting laboratory. 

Sourcehttp://www.dailymail.co.uk/sciencetech/article-4448508/NASA-investigate-unknown-fungus-space-station.html


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